Frequently asked questions

1. Where are we located?

2. How to contact the GML?

3. What are the products available at the GML?

4.What are the recommended protocols for microarray work?

5. Why does the SUBMIT button not work?

6. How to place microarray orders?

7. What is the criteria for RNA samples?

8. What if my RNA for labeling is limited?

9. How long to complete a microarray order?

10. What is the cost of the various services offered at GML?

 

 

1. Where are we located?                                                                          

Genomics and Microarray Laboratory

Department of Environmental Health

Kettering Laboratory, Room 336

3223 Eden Avenue

University of Cincinnati Medical Center

Cincinnati, OH  45267-0056

 

2.How to contact the GML?                                                                      

Saikumar Karyala :  (513)558-4764; microarray@uc.edu

GML:  (513)558-4764; microarray@uc.edu

 

3. What are the products available at the GML?                                      

Click here to see the products

 

4.What are the recommended protocols for microarray work?               

Click here to see the recommended protocols

 

5.Why does the SUBMIT button not work ?                                             

An outdated version of Microsoft Explorer or Netscape Navigator was used.  Microsoft Explorer version 5.0 or higher or Netscape Navigator version 6.0 or higher is required.

Click here to download the latest version of Microsoft Explorer 

Click here to download the latest version of Netscape Navigator

 

6. How to place microarray orders?                                                         

Click here to place orders

 

7.What is the criteria for RNA samples?                                 

The quality of the RNA for labeling is critical for successful microarrays.  The best current way to determine RNA quality is to obtain RNA profiles using the Agilent Bioanalyzer 2100.

The RNA must meet certain minimum criteria as determined from the Agilent Bioanalyzer analysis. 

Click here for more details

8. What if my RNA amount for labeling is limited?                                  

We recommend 20mg per fluorescence labeling reaction.  We can successfully label 10mg per reaction but there is some loss of fluorescence intensity.  Other methods are available for labeling lesser amounts of total RNA, e.g., the labeling kit from Arcturus.  A labeling option will be applied after consultation with the investigator.

 

9. How long to complete a microarray order?                                          

Three to 4 weeks after submitting the RNA for labeling.

 

10.What is the cost of various services offered at GML?                        

Click here to see the Price List